Kutateladze, Tamara / T. და Gabriadze, Inga / I. და Datukishvili, Nelly / N. და Karseladze, Marina / M. და Zaalishvili , Tengiz / T.
(2013)
Screening of genetically modified plants by duplex PCR.
Annals of Agrarian Science, 11 (1).
С. 76-79.
ISSN 1512-1887
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რეზიუმე
Two duplex polymerase chain reactions were developed for screening genetically modified plants. The certified reference materials as dried powders containing 0-5% Roundup Ready soybean and maize MON-810 were used for optimization and validation of PCR methods. The genomic DNAs were extracted by Qiagen DNeasy plant mini kit and analysed using species-specific and GMO-specific uniplex PCR. The PCR products were evaluated by agarose gel electrophoresis. Different combinations of the GMO-specific primers were verified for their suitability for duplex PCR. Two duplex PCRs were developed and optimized, that allow simultaneous detection of two common transgenic elements, such as: cauliflower mosaic virus (CaMV) 35S promoter and Agrobacterium tumefaciens nopaline synthase (NOS) terminator. The developed duplex PCRs are effective tools for rapid and cheap screening of transgenic plants.
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Screening of genetically modified plants by duplex PCR. (deposited 23 იანვარი 2014 19:22)
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