Screening of genetically modified plants by duplex PCR

Kutateladze, Tamara / T. and Gabriadze, Inga / I. and Datukishvili, Nelly / N. and Karseladze, Marina / M. and Zaalishvili , Tengiz / T. (2013) Screening of genetically modified plants by duplex PCR. Annals of Agrarian Science, 11 (1). pp. 76-79. ISSN 1512-1887

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Abstract

Two duplex polymerase chain reactions were developed for screening genetically modified plants. The certified reference materials as dried powders containing 0-5% Roundup Ready soybean and maize MON-810 were used for optimization and validation of PCR methods. The genomic DNAs were extracted by Qiagen DNeasy plant mini kit and analysed using species-specific and GMO-specific uniplex PCR. The PCR products were evaluated by agarose gel electrophoresis. Different combinations of the GMO-specific primers were verified for their suitability for duplex PCR. Two duplex PCRs were developed and optimized, that allow simultaneous detection of two common transgenic elements, such as: cauliflower mosaic virus (CaMV) 35S promoter and Agrobacterium tumefaciens nopaline synthase (NOS) terminator. The developed duplex PCRs are effective tools for rapid and cheap screening of transgenic plants.

Item Type: Article
Subjects: S Agriculture > SB Plant culture
Divisions: Faculties/Schools > Faculty of Arts and Sciences
Depositing User: Prof. Nelly / N. Datukishvili
Date Deposited: 14 Apr 2015 12:33
Last Modified: 14 Apr 2015 12:33
URI: http://eprints.iliauni.edu.ge/id/eprint/1941

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