Application of PCR-based methods for rapid detection of corn ingredients in processed foods

Gabriadze, Inga / I. and Kutateladze, Tamara / T. and Vishnepolsky, Boris / B. and Karseladze, Marina / M. and Datukishvili, Nelly / N. (2014) Application of PCR-based methods for rapid detection of corn ingredients in processed foods. International Journal of Nutrition and Food Sciences, 3 (3). pp. 199-202. ISSN 2327-2694 (Print); 2327-2716 (Online)

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Abstract

A rapid and accurate detection of corn (Zea mays L.) ingredients in processed foods is important for food safety and quality assurance. This study aimed to develop PCR-based approach for fast screening of the corn in foodstuffs. To this purpose a new PCR-based DNA marker specific to the corn zein gene was developed, three uniplex PCR methods and one triplex PCR system targeting invertase and zein genes were compared. Different corn-derived foodstuffs such as: flour, chips, flakes and snacks were investigated. Analysis of PCR products by agarose gel electrophoresis demonstrated that multiplex PCR method represents the most reliable and rapid tool for identification of corn ingredients in highly processed foods.

Item Type:	Article
Uncontrolled Keywords:	Corn, Processed Foods, Genomic DNA Extraction, PCR Analysis
Subjects:	S Agriculture > SB Plant culture
Divisions:	Faculties/Schools > School of Natural Sciences and Engineering
Depositing User:	Prof. Nelly / N. Datukishvili
Date Deposited:	14 Apr 2015 12:33
Last Modified:	14 Apr 2015 12:33
URI:	http://eprints.iliauni.edu.ge/id/eprint/1943

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