The actin binding protein destrin is associated with growth and perineural invasion of pancreatic cancer.

Klose, Theresa and Abiatari, Ivane and Samkharadze, Tamar and De Oliveira, Tiago and Jäger, Carsten and Kiladze, Merab and Valkovskaya, Nataliya and Friess, Helmut and Michalski, Christoph W and Kleeff, Jörg (2012) The actin binding protein destrin is associated with growth and perineural invasion of pancreatic cancer. Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], 12 (4). pp. 350-7. ISSN 1424-3911

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Abstract

BACKGROUND/OBJECTIVES The small actin-binding protein destrin is one of the key regulators involved in remodeling of the actin cytoskeleton, a process crucial for cytokinesis, cell migration and polarized cell growth as well as for cancer cell migration and invasion. METHODS A novel ex vivo nerve invasion model mirroring perineural cancer cell invasion as a key feature of pancreatic ductal adenocarcinoma has been previously established. Using this model, highly nerve-invasive clones of human pancreatic cancer cell lines have been obtained. Genome-wide transcriptional analyses of these cells revealed up-regulation of destrin in highly versus lowly nerve-invasive pancreatic cancer cells. RESULTS Increased expression of destrin in these nerve-invasive cells was validated using quantitative RT-PCR and immunoblotting; concomitant changes in cell morphology were demonstrated using immunofluorescence analysis. Silencing of destrin by two specific siRNA oligonucleotides in Panc-1 pancreatic cancer cells decreased invasiveness and migration, and reduced proliferation of these cells. CONCLUSIONS Destrin is upregulated in nerve-invasive pancreatic cancer cells and its expression might be related to perineural invasiveness.

Item Type: Article
Subjects: R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Depositing User: Dr Ivane Abiatari
Date Deposited: 30 Apr 2015 05:27
Last Modified: 30 Apr 2015 05:27
URI: http://eprints.iliauni.edu.ge/id/eprint/2222

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