Thyroid hormones differentially regulate phosphorylation of ERK and Akt via integrin αvβ3 receptor in undifferentiated and differentiated PC-12 cells.

Barbakadze, Tamar და Natsvlishvili, Nino და Mikeladze, David (2013) Thyroid hormones differentially regulate phosphorylation of ERK and Akt via integrin αvβ3 receptor in undifferentiated and differentiated PC-12 cells. Cell biochemistry and function. ISSN 1099-0844

სრული ტექსტი ამ საცავიდან ხელმიუწვდომელია.

რეზიუმე

The effects of 3,5,3'-triiodo-l-thyronine (T3) and l-thyroxine (T4) on the integrin αvβ3 receptor of thyroid hormones (TH) were investigated in pheochromocytoma PC-12 cells. Differentiation was induced by treatment of PC-12 cells with fisetin and the levels of phosphorylated extracellular signal-regulated kinase (ERK) and Akt in cytoplasm, as well as the content of FoxO6 transcription factor in nuclei was analysed in undifferentiated and differentiated conditions. We have found that in undifferentiated PC-12 cells, tetraiodothyroacetic acid (TETRAC), a known inhibitor of binding of T4 and T3 to plasma membrane integrin αvβ3 receptor inhibits T4-dependent phosphorylation of ERK, whereas in differentiated PC-12 cells, TETRAC abolishes the effect of T3. In undifferentiated PC-12 cells, both TH increase the level of p-Akt, and this enhancement is not sensitive to TETRAC. In differentiated PC-12 cells, both TH increase the level of p-Akt; however, only T3-dependent activation of Akt is sensitive to the TETRAC. Furthermore, our results have shown that in differentiated PC-12 cells, the expression of FoxO6 was higher than in undifferentiated PC-12 cells, and this elevation has not changed under the action of TH. Only in undifferentiated PC-12 cells the T3-dependent expression of FoxO6 was sensitive to the TETRAC. We propose that PC-12 cells contain integrin αvβ3 receptor, which T3 and T3/T4 sites are differentially regulated by TH in undifferentiated and differentiated conditions. Copyright © 2013 John Wiley & Sons, Ltd.

ობიექტის ტიპი:	სტატია
თემატიკა:	Q Science > QH Natural history > QH301 Biology
განმათავსებელი მომხმარებელი:	Prof. Tamar Barbakadze
განთავსების თარიღი:	22 იანვარი 2014 05:53
ბოლო ცვლილება:	22 იანვარი 2014 05:53
URI:	http://eprints.iliauni.edu.ge/id/eprint/361

ამ მასალაზე ხელმისაწვდომი ვერსია

• Thyroid hormones differentially regulate phosphorylation of ERK and Akt via integrin αvβ3 receptor in undifferentiated and differentiated PC-12 cells. (deposited 22 იანვარი 2014 05:53) [ასახულია მიმდინარე დროს]
  • Thyroid hormones differentially regulate phosphorylation of ERK and Akt via integrin αvβ3 receptor in undifferentiated and differentiated PC-12 cells. (deposited 22 ივნისი 2015 05:35)

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