Development of triplex PCR for simultaneous detection of maize, wheat and soybean

Kutateladze, Tamara / T. and Gabriadze, Inga / I. and Vishnepolsky, Boris / B. and Karseladze, Marina / M. and Datukishvili, Nelly / N. (2013) Development of triplex PCR for simultaneous detection of maize, wheat and soybean. Food Control, 34 (2). pp. 698-702. ISSN 0956-7135

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Abstract

A reliable and fast detection of important food plants, such as maize (Zea mays L.), wheat (Triticum aestivum L.), and soybean (Glycine max L.) is of particular interest for food authenticity and safety assessment. In this study, the novel multiplex polymerase chain reaction (PCR) method was developed for the rapid qualitative detection of soybean, maize and wheat. To this purpose, new soybean-specific and maize-specific PCR primers were designed. Their specificity was assayed by uniplex PCRs with different plant species, namely maize, soybean, wheat, oats (Avena sativa), and barley (Hordeum vulgare L). Gel electrophoresis of the amplification products demonstrated high specificity of both primer pairs for identification of relevant species. Subsequently, based on the developed DNA markers, the species-specific triplex PCR targeting maize invertase gene, soybean lectin gene and wheat low-molecular-weight glutenin subunit was developed and optimized for simultaneous identification of these three plant species. The developed PCR method enables specific, effective and rapid detection of maize, wheat and soybean and may be used for food analysis.

Item Type:	Article
Uncontrolled Keywords:	Food plants, species detection, DNA analysis, multiplex PCR
Subjects:	S Agriculture > SB Plant culture
Divisions:	Faculties/Schools > Faculty of Arts and Sciences
Depositing User:	Prof. Nelly / N. Datukishvili
Date Deposited:	14 Apr 2015 12:32
Last Modified:	14 Apr 2015 12:32
URI:	http://eprints.iliauni.edu.ge/id/eprint/1957

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