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Comparison of screening methods for reliable detection of genetically modified organisms

Datukishvili, Nelly / N. and Kutateladze, Tamara / T. and Karseladze, Marina / M. and Gabriadze, Inga / I. (2009) Comparison of screening methods for reliable detection of genetically modified organisms. Proceedings of the Georgian Academy of Sciences, Biological Series, 7 (3-4). pp. 4-8. ISSN 1512 - 2123

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Abstract

Three PCR primer pairs suitable for common regulatory elements of genetically modified organisms (GMOs) were compared for selection the most effective screening method for GMO. Two of the primer pairs such as: 35S1/35S2 and 35S-cf3/35S-cr4 are specific to 35S promoter from Cauliflower Mosaic virus and one primer pair NOS1/NOS2 is specific to the NOS terminator from Agrobacterium tumefaciens. The Roundup Ready soybean was used as GM material. The comparison of the primer pairs was carried out in two different PCR conditions PCR1 and PCR2. The results obtained exhibited 35S promoter specific primer pair 35S-cf3/35S-cr4 as the most specific, sensitive and efficient tool for GMO screening in the both PCR conditions applied.

Item Type: Article
Uncontrolled Keywords: Screening of GMO, genetically modified organisms (GMOs), PCR analysis, regulatory elements
Subjects: S Agriculture > SB Plant culture
Divisions: Faculties/Schools > Faculty of Arts and Sciences
Depositing User: Prof. Nelly / N. Datukishvili
Date Deposited: 14 Apr 2015 12:33
Last Modified: 14 Apr 2015 12:33
URI: http://eprints.iliauni.edu.ge/id/eprint/1938

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