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Kotetishvili, M and Stine , OC and Chen , Y and Kreger, A and Sulakvelidze, A and Sozhamannan, S and Morris, JG Jr (2003) Multilocus sequence typing has better discriminatory ability for typing Vibrio cholerae than does pulsed-field gel electrophoresis and provides a measure of phylogenetic relatedness. J Clin Microbiol.
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Abstract
Twenty-two Vibrio cholerae isolates, including some from "epidemic" (O1 and O139) and "nonepidemic" serogroups, were characterized by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) by using three housekeeping genes, gyrB, pgm, and recA; sequence data were also obtained for the virulence-associated genes tcpA, ctxA, and ctxB. Even with the small number of loci used, MLST had better discriminatory ability than did PFGE. On MLST analysis, there was clear clustering of epidemic serogroups; much greater diversity was seen among tcpA- and ctxAB-positive V. cholerae strains from other, nonepidemic serogroups, with a number of tcpA and ctxAB alleles identified.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | მამუკა კოტეტიშვილი |
| Date Deposited: | 11 Mar 2015 05:57 |
| Last Modified: | 24 Apr 2015 09:15 |
| URI: | http://eprints.iliauni.edu.ge/id/eprint/1767 |
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