Collagen–DNA Complex

Svintradze , David V. და Mrevlishvili, George M. და Metreveli, Nunu და Jariashvili , Ketevan და Namicheishvili, Luisa და Skopinska, Joana და Sionkowska , Alina (2008) Collagen–DNA Complex. Biomacromolecules, 9 (1). С. 21-28.

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Previously presented models of collagen–DNA7 and collagen–siRNA8 complexes point to a general description of delivery systems and indicate to what specific topology that system should be equipped with to effectively deliver the gene into the living body via in vivo and in vitro injection. We focused our interest on the nature of collagen–DNA complex structure and the molecular and environmental determinants of the self-association processes of collagen with the presence of DNA. In this aspect, the self-association of collagen–DNA complex offers an opportunity to characterize a unique system, which may be related to the general mechanisms of self-association of fiber macromolecules by water bridges. For characterizing the collagen–DNA interaction, we used FTIR-ATR, NMR, and AFM experiments done on a separate collagen film, DNA film, and on the peptide–DNA aqueous solution. We demonstrate that collagen–DNA spontaneously forms self-assembling complex systems in aqueous solution. Such self-association of the complex could be induced by electrostatic interactions between neutral collagen cylinders, having strong dipole moment, and negatively charged DNA cylinders. A final complex could be formed by hydrogen bonds between specified donor groups of collagen and phosphate acceptor groups of DNA. According to FTIR measurements, a collagen triple helix should not change global conformation during collagen–DNA complex formation.

ობიექტის ტიპი: სტატია
თემატიკა: Q Science > QC Physics
Q Science > QH Natural history > QH301 Biology
ქვეგანყოფილება: Institutes > Institute of Biophysics
განმათავსებელი მომხმარებელი: პროფესორი ნუნუ მეტრეველი
განთავსების თარიღი: 11 თებერვალი 2014 12:25
ბოლო ცვლილება: 12 მარტი 2014 15:11

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