UV–vis and FT-IR spectra of ultraviolet irradiated collagen in the presence of antioxidant ascorbic acid

Metreveli, Nunu და Jariashvili, Ketevan და Namicheishvili, Louisa და Svintradzea, David და Chikvaidze, Eduard და Sionkowska, Alina და Skopinska, Joanna (2010) UV–vis and FT-IR spectra of ultraviolet irradiated collagen in the presence of antioxidant ascorbic acid. Ecotoxicology and Environmental Safety, 73 (3). С. 448-455.

ტექსტი 20036425 - გამოქვეყნებული ვერსია Download (76kB)

რეზიუმე

The influence of deleterious UV radiation on collagen molecules in the absence and presence of ascorbic acid using UV–vis and FT-IR spectroscopy has been studied. Intensity of UV–vis absorption spectrum of collagen with a maximum at 275 nm due to the aromatic residues (tyrosine and phenylalanine) increases with the increasing dose of UV radiation. This effect is significantly hindered in the presence of antioxidant ascorbic acid. Intensities of FT-IR bands (amide A, B, I and II) of collagen decrease with the increase of the UV radiation dosage. Intensities of bands are also decreased in the presence of ascorbic acid. Results suggest that increasing the concentration of ascorbic acid increases the photo-stability of collagen, and the collagen becomes less sensitive to UV radiation. It is possible that hydrogen bonds form between the groups N–H of collagen and C=O of ascorbic acid. It is believed that under UV radiation free radicals appear in acid soluble collagen and resulting in photodegradation of the macromolecule restore due to the ability of ascorbic acid donating one or two electrons. Increasing the dose of radiation causes more molecules of ascorbic acid to slow down, and their antioxidant effect is diminished accordingly.

ობიექტის ტიპი:	სტატია
თემატიკა:	Q Science > QC Physics Q Science > QH Natural history > QH301 Biology
ქვეგანყოფილება:	Institutes > Institute of Biophysics
განმათავსებელი მომხმარებელი:	პროფესორი ნუნუ მეტრეველი
განთავსების თარიღი:	11 თებერვალი 2014 08:33
ბოლო ცვლილება:	11 თებერვალი 2014 08:33
URI:	http://eprints.iliauni.edu.ge/id/eprint/961

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