18 Georgian and 4 West-European cultivars of V. vinifera, 31 individuals of wild V. vinifera subsp. sylvestris from different regions of Georgia and 2 samples of V. riparia were genotyped at eight nuclear microsatellite loci to investigate genetic relationships between cultivated and wild grapevines. This study showed strong genetic similarities between cultivated Georgian grapes and wild grapevine. Oldest Georgian cultivars – ‘Krikina’, ‘Saperavi’ and ‘Uchakhardani’ were unified in one cluster with local wild grapes. Very high value of genetic similarity (0.9) was detected between the old Georgian cultivar ‘Rkatsiteli’ and wild grapevine from the Lekhura gorge in Shida Kartli. Consequently, the admixture found among local Georgian cultivars and wild grapevine indicates the possibility that these cultivars are derived from ancestral domestication events of local wild grapevine.