485 7 archive 90 disk0/00/00/04/85 2014-01-23 19:22:47 2014-01-23 19:22:47 2014-01-23 19:22:47 article no_search neli_datukishvili@iliauni.edu.ge Kutateladze Tamara / T. Gabriadze Inga / I. Datukishvili Nelly / N. neli_datukishvili@iliauni.edu.ge Karseladze Marina / M. Zaalishvili Tengiz / T. pub SB fac_med none Two duplex polymerase chain reactions were developed for screening genetically modified plants. The certified reference materials as dried powders containing 0-5% Roundup Ready soybean and maize MON-810 were used for optimization and validation of PCR methods. The genomic DNAs were extracted by Qiagen DNeasy plant mini kit and analysed using species-specific and GMO-specific uniplex PCR. The PCR products were evaluated by agarose gel electrophoresis. Different combinations of the GMO-specific primers were verified for their suitability for duplex PCR. Two duplex PCRs were developed and optimized, that allow simultaneous detection of two common transgenic elements, such as: cauliflower mosaic virus (CaMV) 35S promoter and Agrobacterium tumefaciens nopaline synthase (NOS) terminator. The developed duplex PCRs are effective tools for rapid and cheap screening of transgenic plants. 2013-01 published Annals of Agrarian Science 11 1 76-79 TRUE 1512-1887 http://www.agrscience.ge/content/content11_1.pdf http://www.agrscience.ge/Agrarian%20Science-main.htm pub Shota Rustaveli National science Foundation Grant # GRANT/ST 09_262_8-150