Manumycin restores the levels of cortical homocysteine, methionine and cysteine changed in ischaemia-evoked animals

406 10 1521 1 4423 document 1521 2004.pdf application/pdf f909ed0c0abc260471f272a5e3679616 MD5 160204 2014-01-22 10:06:22 http://eprints.iliauni.edu.ge/406/1/2004.pdf 406 1 1 application/pdf text ge public

2004.pdf

1523 2 4435 document 1523 lightbox.jpg image/png 6933fa596d9df1bed5a7319c996744c8 MD5 63338 2014-01-22 10:16:41 http://eprints.iliauni.edu.ge/406/3/lightbox.jpg 406 3 3 image/png other Thumbnails conversion from text to thumbnail_lightbox ge public

lightbox.jpg

preview.jpg

medium.jpg

small.jpg

indexcodes.txt

http://eprints.org/relation/isVersionOf http://eprints.iliauni.edu.ge/id/document/1521 http://eprints.org/relation/isVolatileVersionOf http://eprints.iliauni.edu.ge/id/document/1521 http://eprints.org/relation/isIndexCodesVersionOf http://eprints.iliauni.edu.ge/id/document/1521 archive 61 disk0/00/00/04/06 2014-01-22 14:34:52 2014-01-25 08:20:30 2014-01-22 14:34:52 article show Shanshiashvili Lali lali_shanshiashvili@iliauni.edu.ge Sekoyan E. Mikeladze David Manumycin restores the levels of cortical homocysteine, methionine and cysteine changed in ischaemia-evoked animals pub QH301 QP R1 ins10 public Reactive oxygen species (ROS) formed during ischaemia can activate the Ras signalling system, which amplifies the intracellular formation of ROS. Ras proteins induce senescence by altering the intracellular levels of ROS which leads to the impairment of homocysteine metabolism. The effects of the farnesyltransferase inhibitor, manumycin, were examined on ischaemia-evoked changed sulfur-containing amino acids. A rat four-vessel occlusion model was utilized to examine the effects of manumycin on cortical levels of homocysteine, methionine and cysteine. Cerebral ischaemia was induced by occlusion of the carotid arteries for 20 min in anaesthetized animals. Intraperitoneal injection of manumycin A (3 mg/kg body weight) was carried out 2 hours before anaesthesia. It was found that after ischaemia the quantities of methionine and homocysteine were decreased, whereas the amount of cysteine was increased. In the rats pretreated with manumycin, cerebral ischaemia does not change the content of methionine, homocysteine or cysteine. These results suggest that ischaemiainduced oxidative stress, acting through Ras-dependent processes, change homocysteine metabolism in the direction of trans-sulfuration. Keywords: homocysteine, ischaemia, Ras 2004 Journal of Biological Physics and Chemistry 4 Collegium Basilea & AMSI 197-200 TRUE INTAS grant (no. 2001- 0666)