Neuronal Porosome in the Rat and Cat Brain

258 8 953 1 2918 document 953 BCT69.pdf application/pdf 9e32e1af02ba589c04d8d0b1983fa644 MD5 369301 2014-01-20 10:26:20 http://eprints.iliauni.edu.ge/258/1/BCT69.pdf 258 1 1 application/pdf text ge public

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965 2 2951 document 965 lightbox.jpg image/png 91caab01e074e43620e7a1178e68cb06 MD5 57620 2014-01-20 10:48:28 http://eprints.iliauni.edu.ge/258/2/lightbox.jpg 258 2 2 image/png other Thumbnails conversion from text to thumbnail_lightbox ge public

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http://eprints.org/relation/isVersionOf http://eprints.iliauni.edu.ge/id/document/953 http://eprints.org/relation/isVolatileVersionOf http://eprints.iliauni.edu.ge/id/document/953 http://eprints.org/relation/issmallThumbnailVersionOf http://eprints.iliauni.edu.ge/id/document/953 archive 87 disk0/00/00/02/58 2014-01-20 10:47:17 2015-04-16 08:32:39 2014-01-20 10:47:17 article show Okuneva VG vera_okuneva@yahoo.com Japaridze NG nadia_japaridze@yahoo.com Kotaria Nato N_KOTARIA@YAHOO.COM Zhvania Mzia G mzia_zhvania@iliauni.edu.ge Zhvania MG Okuneva VG - PhD student Neuronal Porosome in the Rat and Cat Brain pub QP ins10 public Abstract—It is well established that during cell secretion, membrane bound secretory vesicles dock and fuse at the base of supramolecular cup-shaped structures at the cell plasma membrane called “porosomes”, to expel intra vesicular contents to the outside. In neurons, it has been demonstrated that 12–17 nm cup shaped lipoprotein structure possessing a central plug are present at the presynaptic membrane, where 50 nm in diameter synaptic vesicles transiently dock and fuse to release neurotransmitters. In the past decade, the neuronal porosome has been isolated and its major chemical composition determined. Additionally, the porosome has been both structurally and functionally reconstituted into artificial lipid membrane, establish ing its role as the secretory portal in neurons. Studies utilizing atomic force and electron microscopy, combined with electron density and 3D contour mapping, provide at the nanoscale, the structure and assembly of proteins within the neuronal porosome. In the current study, ultrahigh resolution imaging of the presynaptic membrane of isolated brains from both rats and cats, demonstrate for the first time, the presence of neuronal porosomes in cat brain, and further confirms the presence of porosomes at the presynaptic membrane in rat brain synaptosomes. Results from the present study further confirm the cup shaped morphology of porosomes in the rat brain, and demonstrates their similar shape and size in the cat nerve terminal. The study also demonstrates for the first time, the universal presence of similar porosomes in different species of mam mals. 2012 Cell and Tissue Biology 6 1 Pleiades Publishing LTD 69-72 TRUE 1990-519X