Membrane-directed molecular assembly of the neuronal SNARE complex Won

237 12 923 1 2793 document 923 JCMM-2011(s)[1].pdf application/pdf 4374e80942f370b807aafc3a90a12a97 MD5 853953 2014-01-20 08:39:38 http://eprints.iliauni.edu.ge/237/1/JCMM-2011%28s%29%5B1%5D.pdf 237 1 1 application/pdf text ge public

JCMM-2011(s)[1].pdf

924 2 2805 document 924 lightbox.jpg image/png 73c57e21728913009c7b6e2aeba79bbc MD5 45074 2014-01-20 08:50:01 http://eprints.iliauni.edu.ge/237/2/lightbox.jpg 237 2 2 image/png other Thumbnails conversion from text to thumbnail_lightbox ge public

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http://eprints.org/relation/isVersionOf http://eprints.iliauni.edu.ge/id/document/923 http://eprints.org/relation/isVolatileVersionOf http://eprints.iliauni.edu.ge/id/document/923 http://eprints.org/relation/issmallThumbnailVersionOf http://eprints.iliauni.edu.ge/id/document/923 archive 87 disk0/00/00/02/37 2014-01-20 08:50:21 2015-04-16 08:34:14 2014-01-20 08:50:21 article show Cho Won Jin Lee Jin-Sook Zhang Lei Ren Gang Shin Leah Manke Charles W Potoff Jeffrey Kotaria Nato N_KOTARIA@YAHOO.COM Zhvania Mzia G mzia_zhvania@iliauni.edu.ge Jena Bhanu P bjena@med.wayne.edu Membrane-directed molecular assembly of the neuronal SNARE complex Won pub QP ins10 public Since the discovery and implication of N-ethylmaleimide-sensitive factor (NSF)-attachment protein receptor (SNARE) proteins in membrane fusion almost two decades ago, there have been significant efforts to understand their involvement at the molecular level. In the current study, we report for the first time the molecular interaction between full-length recombinant t-SNAREs and v-SNARE present in opposing liposomes, leading to the assembly of a t-/v-SNARE ring complex. Using high-resolution electron microscopy, the electron density maps and 3D topography of the membrane-directed SNARE ring complex was determined at nanometre resolution. Similar to the t-/v-SNARE ring complex formed when 50 nm v-SNARE liposomes meet a t-SNARE-reconstituted planer membrane, SNARE rings are also formed when 50 nm diameter isolated synaptic vesicles (SVs) meet a t-SNARE-reconstituted planer lipid membrane. Furthermore, the mathematical prediction of the SNARE ring complex size with reasonable accuracy, and the possible mechanism of membrane-directed t-/v-SNARE ring complex assembly, was determined from the study. Therefore in the present study, using both lipososome-reconstituted recombinant t-/v-SNARE proteins, and native v-SNARE present in isolated SV membrane, the membranedirected molecular assembly of the neuronal SNARE complex was determined for the first time and its size mathematically predicted. These results provide a new molecular understanding of the universal machinery and mechanism of membrane fusion in cells, having fundamental implications in human health and disease. 2011 Journal of Cellular and Molecular Medicine 15 1 Wiley 31-37 TRUE 1582-4934