The effect of kainic acid on hippocampal dendritic spine motility at the early and late stages of brain development

233 8 911 1 2771 document 911 Micron.pdf application/pdf 9b06486321b8127c127892adc55a758d MD5 312213 2014-01-20 08:21:06 http://eprints.iliauni.edu.ge/233/1/Micron.pdf 233 1 1 application/pdf text ge public

Micron.pdf

917 2 2785 document 917 lightbox.jpg image/png ca7d803bbfb85fdc1f98aef3cb748dd8 MD5 23433 2014-01-20 08:31:26 http://eprints.iliauni.edu.ge/233/2/lightbox.jpg 233 2 2 image/png other Thumbnails conversion from text to thumbnail_lightbox ge public

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http://eprints.org/relation/isVersionOf http://eprints.iliauni.edu.ge/id/document/911 http://eprints.org/relation/isVolatileVersionOf http://eprints.iliauni.edu.ge/id/document/911 http://eprints.org/relation/issmallThumbnailVersionOf http://eprints.iliauni.edu.ge/id/document/911 archive 87 disk0/00/00/02/33 2014-01-20 08:45:38 2014-01-23 19:25:32 2014-01-20 08:45:38 article show Lortdkipanidze Tamar tamar_lordkipanidze@iliauni.edu.ge Bikashvili Tamar takobikashvili@yahoo.com Japaridze Nadezhda T nadia_japaridze@yahoo.com Zhvania Mzia G mzia_zhvania@iliauni.edu.ge Lordkipanidze Tamar The effect of kainic acid on hippocampal dendritic spine motility at the early and late stages of brain development pub QP ins10 public Dendrites and spines undergo dynamic changes in physiological conditions, such as learning and memory, and in pathological conditions, such as epilepsy. Abnormalities in dendritic spines have commonly been observed in brain specimens from epilepsy patients and animal models of epilepsy. However, the functional implications and clinical consequences of this dendritic pathology for epilepsy are uncertain. Motility of dendritic spines and axonal filopodia has been recently discovered by the advanced imaging techniques, and remains to a large degree an exciting phenomenology in search of function. Here we demonstrate the effect of kainic acid (KA), which is a structural analog of glutamate, on dendritic spine motility in hippocampal CA1 area at the different stages of brain development. In order to reveal the changes that take place in spine and filopodial motility in the epileptic model of brain, time-lapse imaging of acute hippocampal slices treated with various concentrations of KA after different incubation time points was performed. The effects of KA exposure were tested on the slices from young (postnatal day (P)7–P10) and adolescent (P28–P30) Thy1-YFPH transgenic mice. Slices were treated with either 50 �M or 100 �M of KA, for either 30 or 100 min. The results obtained in our experiments show diverse effects of KA in 2 different age groups. According to our results, 100 �M/100 min KA treatment increases spine motility at early stage of brain development (P10) by 41.5%, while in P30 mice spine motility is increased only by 3%. Our findings also indicate that effect of KA on hippocampal dendritic spine motility is predominantly time- rather than concentration-dependent. 2013 Micron 49 Elsevier 28-32 TRUE