2222 10 9641 1 26764 document 9641 DSTN.pdf application/pdf a6a51860cdad9cfda21c56b3697ab0e4 MD5 1232102 2015-04-29 13:19:19 http://eprints.iliauni.edu.ge/2222/1/DSTN.pdf 2222 1 1 application/pdf text en public
DSTN.pdf
 archive 207 disk0/00/00/22/22 2015-04-30 05:27:14 2015-04-30 05:27:14 2015-04-30 05:27:14 article show Klose Theresa Abiatari Ivane ivane.abiatari@iliauni.edu.ge Samkharadze Tamar De Oliveira Tiago Jäger Carsten Kiladze Merab Valkovskaya Nataliya Friess Helmut Michalski Christoph W Kleeff Jörg The actin binding protein destrin is associated with growth and perineural invasion of pancreatic cancer. pub RC0254 public BACKGROUND/OBJECTIVES The small actin-binding protein destrin is one of the key regulators involved in remodeling of the actin cytoskeleton, a process crucial for cytokinesis, cell migration and polarized cell growth as well as for cancer cell migration and invasion. METHODS A novel ex vivo nerve invasion model mirroring perineural cancer cell invasion as a key feature of pancreatic ductal adenocarcinoma has been previously established. Using this model, highly nerve-invasive clones of human pancreatic cancer cell lines have been obtained. Genome-wide transcriptional analyses of these cells revealed up-regulation of destrin in highly versus lowly nerve-invasive pancreatic cancer cells. RESULTS Increased expression of destrin in these nerve-invasive cells was validated using quantitative RT-PCR and immunoblotting; concomitant changes in cell morphology were demonstrated using immunofluorescence analysis. Silencing of destrin by two specific siRNA oligonucleotides in Panc-1 pancreatic cancer cells decreased invasiveness and migration, and reduced proliferation of these cells. CONCLUSIONS Destrin is upregulated in nerve-invasive pancreatic cancer cells and its expression might be related to perineural invasiveness. 2012 published Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 12 4 350-7 TRUE 1424-3911