2210 9 9647 1 26782 document 9647 Abiatari et al_Hsulf-1 regulates growth and invasion of pancreatic cancer cells.pdf application/pdf 518ec8a3d0827d0bf1e4f085416b8eed MD5 3661382 2015-04-29 13:24:57 http://eprints.iliauni.edu.ge/2210/1/Abiatari%20et%20al_Hsulf-1%20regulates%20growth%20and%20invasion%20of%20pancreatic%20cancer%20cells.pdf 2210 1 1 application/pdf text en public
Abiatari et al_Hsulf-1 regulates growth and invasion of pancreatic cancer cells.pdf
 archive 207 disk0/00/00/22/10 2015-04-30 05:28:59 2015-04-30 05:28:59 2015-04-30 05:28:59 article show Abiatari Ivane ivane.abiatari@iliauni.edu.ge Kleeff J Li J Felix K Büchler M W Friess H Hsulf-1 regulates growth and invasion of pancreatic cancer cells. pub RC0254 public BACKGROUND Hsulf-1 is a newly identified enzyme with arylsulphatase activity that can regulate the sulphation state of cell-surface heparan sulphate proteoglycans (HSPGs). In vitro overexpression of this enzyme in pancreatic cancer cells decreases responsiveness to fibroblastic growth factor-2, as Hsulf-1 is up regulated in primary pancreatic adenocarcinoma. AIM To further analyse the functions of the Hsulf-1 enzyme in vitro and in vivo with respect to growth, invasion and tumorigenicity. METHODS AND RESULTS Transfection of Panc-1 pancreatic cancer cells with a full-length Hsulf-1 expression vector resulted in increased invasiveness and adhesiveness. An in vivo xenograft nude mouse tumour model showed a markedly reduced growth potential of Hsulf-1-expressing Panc-1 cells, which correlated with a considerably lower proliferation rate. Hsulf-1-positive nude mouse tumours showed better development of interstitial matrix structures, with increased blood vessel density in these tumours. In an orthotopic model, Hsulf-1-positive tumours exhibited enhanced local invasiveness. In human primary pancreatic cancers there was strong staining for sulphated HSPGs, which was markedly reduced in metastatic tissue samples. CONCLUSION Hsulf-1-mediated desulphation of HSPGs reduces the growth ability of Panc-1 pancreatic cancer cells, but increases the basal invasiveness of these cells, suggesting an important role of this enzyme in pancreatic cancer progression. 2006-10 published Journal of clinical pathology 59 10 1052-8 TRUE 0021-9746