1941 5 8629 1 23254 document 8629 AAS_2013.pdf application/pdf 36a9cdf06adbe00db3e9e2351109bc11 MD5 3622460 2015-04-07 15:56:03 http://eprints.iliauni.edu.ge/1941/1/AAS_2013.pdf 1941 1 1 application/pdf text en public

AAS_2013.pdf

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http://eprints.org/relation/isVersionOf http://eprints.iliauni.edu.ge/id/document/8629 http://eprints.org/relation/isVolatileVersionOf http://eprints.iliauni.edu.ge/id/document/8629 http://eprints.org/relation/ismediumThumbnailVersionOf http://eprints.iliauni.edu.ge/id/document/8629 8639 2 23270 document 8639 small.jpg image/png 128ad07dd44245f1076a14f67e99c7fb MD5 1930 2015-04-07 16:06:13 http://eprints.iliauni.edu.ge/1941/5/small.jpg 1941 5 5 image/png other Thumbnails conversion from text to thumbnail_small ge public

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http://eprints.org/relation/isVersionOf http://eprints.iliauni.edu.ge/id/document/8629 http://eprints.org/relation/isVolatileVersionOf http://eprints.iliauni.edu.ge/id/document/8629 http://eprints.org/relation/issmallThumbnailVersionOf http://eprints.iliauni.edu.ge/id/document/8629 archive 90 disk0/00/00/19/41 2015-04-14 12:33:18 2015-04-14 12:33:18 2015-04-14 12:33:18 article 485 show Kutateladze Tamara / T. Gabriadze Inga / I. Datukishvili Nelly / N. neli_datukishvili@iliauni.edu.ge Karseladze Marina / M. Zaalishvili Tengiz / T. pub SB fac_med public Two duplex polymerase chain reactions were developed for screening genetically modified plants. The certified reference materials as dried powders containing 0-5% Roundup Ready soybean and maize MON-810 were used for optimization and validation of PCR methods. The genomic DNAs were extracted by Qiagen DNeasy plant mini kit and analysed using species-specific and GMO-specific uniplex PCR. The PCR products were evaluated by agarose gel electrophoresis. Different combinations of the GMO-specific primers were verified for their suitability for duplex PCR. Two duplex PCRs were developed and optimized, that allow simultaneous detection of two common transgenic elements, such as: cauliflower mosaic virus (CaMV) 35S promoter and Agrobacterium tumefaciens nopaline synthase (NOS) terminator. The developed duplex PCRs are effective tools for rapid and cheap screening of transgenic plants. 2013-01 published Annals of Agrarian Science 11 1 76-79 TRUE 1512-1887 pub Shota Rustaveli National science Foundation Grant # GRANT/ST 09_262_8-150