Comparative genomic analyses of the vibrio pathogenicity island and cholera toxin prophage regions in nonepidemic serogroup strains of Vibrio cholerae

1768 8 7464 1 20343 document 7464 1728.full.pdf application/pdf 9edc5487208b615b60d7e995197b3b82 MD5 3821363 2015-03-10 08:51:50 http://eprints.iliauni.edu.ge/1768/1/1728.full.pdf 1768 1 1 application/pdf text ge public

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7471 2 20356 document 7471 lightbox.jpg image/png 9377eb5062284b03d149e7d4796a25b0 MD5 25644 2015-03-10 09:02:14 http://eprints.iliauni.edu.ge/1768/3/lightbox.jpg 1768 3 3 image/png other Thumbnails conversion from text to thumbnail_lightbox ge public

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http://eprints.org/relation/isVersionOf http://eprints.iliauni.edu.ge/id/document/7464 http://eprints.org/relation/isVolatileVersionOf http://eprints.iliauni.edu.ge/id/document/7464 http://eprints.org/relation/issmallThumbnailVersionOf http://eprints.iliauni.edu.ge/id/document/7464 archive 4136 disk0/00/00/17/68 2015-03-11 05:57:48 2015-04-24 09:14:03 2015-03-11 05:57:48 article show Li M Kotetishvili M mamuka_kotetishvili@iliauni.edu.ge Chen Y Sozhamannan S Comparative genomic analyses of the vibrio pathogenicity island and cholera toxin prophage regions in nonepidemic serogroup strains of Vibrio cholerae pub R1 public Two major virulence factors are associated with epidemic strains (O1 and O139 serogroups) of Vibrio cholerae: cholera toxin encoded by the ctxAB genes and toxin-coregulated pilus encoded by the tcpA gene. The ctx genes reside in the genome of a filamentous phage (CTXphi), and the tcpA gene resides in a vibrio pathogenicity island (VPI) which has also been proposed to be a filamentous phage designated VPIphi. In order to determine the prevalence of horizontal transfer of VPI and CTXphi among nonepidemic (non-O1 and non-O139 serogroups) V. cholerae, 300 strains of both clinical and environmental origin were screened for the presence of tcpA and ctxAB. In this paper, we present the comparative genetic analyses of 11 nonepidemic serogroup strains which carry the VPI cluster. Seven of the 11 VPI(+) strains have also acquired the CTXphi. Multilocus sequence typing and restriction fragment length polymorphism analyses of the VPI and CTXphi prophage regions revealed that the non-O1 and non-O139 strains were genetically diverse and clustered in lineages distinct from that of the epidemic strains. The left end of the VPI in the non-O1 and non-O139 strains exhibited extensive DNA rearrangements. In addition, several CTXphi prophage types characterized by novel repressor (rstR) and ctxAB genes and VPIs with novel tcpA genes were found in these strains. These data suggest that the potentially pathogenic, nonepidemic, non-O1 and non-O139 strains identified in our study most likely evolved by sequential horizontal acquisition of the VPI and CTXphi independently rather than by exchange of O-antigen biosynthesis regions in an existing epidemic strain. 2003 Appl Environ Microbiol TRUE