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http://eprints.org/relation/isVersionOf http://eprints.iliauni.edu.ge/id/document/7462 http://eprints.org/relation/isVolatileVersionOf http://eprints.iliauni.edu.ge/id/document/7462 http://eprints.org/relation/ismediumThumbnailVersionOf http://eprints.iliauni.edu.ge/id/document/7462 7468 2 20348 document 7468 small.jpg image/png 462fd621c110823c3e6f59fe7c6b7010 MD5 1839 2015-03-10 08:52:26 http://eprints.iliauni.edu.ge/1767/6/small.jpg 1767 6 6 image/png other Thumbnails conversion from text to thumbnail_small ge public
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http://eprints.org/relation/isVersionOf http://eprints.iliauni.edu.ge/id/document/7462 http://eprints.org/relation/isVolatileVersionOf http://eprints.iliauni.edu.ge/id/document/7462 http://eprints.org/relation/issmallThumbnailVersionOf http://eprints.iliauni.edu.ge/id/document/7462 archive 4136 disk0/00/00/17/67 2015-03-11 05:57:34 2015-04-24 09:15:24 2015-03-11 05:57:34 article show Kotetishvili M mamuka_kotetishvili@iliauni.edu.ge Stine OC Chen Y Kreger A Sulakvelidze A Sozhamannan S Morris JG Jr Multilocus sequence typing has better discriminatory ability for typing Vibrio cholerae than does pulsed-field gel electrophoresis and provides a measure of phylogenetic relatedness pub R1 public Twenty-two Vibrio cholerae isolates, including some from "epidemic" (O1 and O139) and "nonepidemic" serogroups, were characterized by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) by using three housekeeping genes, gyrB, pgm, and recA; sequence data were also obtained for the virulence-associated genes tcpA, ctxA, and ctxB. Even with the small number of loci used, MLST had better discriminatory ability than did PFGE. On MLST analysis, there was clear clustering of epidemic serogroups; much greater diversity was seen among tcpA- and ctxAB-positive V. cholerae strains from other, nonepidemic serogroups, with a number of tcpA and ctxAB alleles identified. 2003 J Clin Microbiol TRUE