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      <item>
        <name>
          <family>Kotetishvili</family>
          <given>M</given>
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      </item>
      <item>
        <name>
          <family>Stine</family>
          <given>OC</given>
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          <family>Kreger</family>
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          <family>Morris</family>
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    <title>Multilocus
sequence typing for characterization of clinical and environmental salmonella
strains</title>
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      <item>R1</item>
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    <full_text_status>public</full_text_status>
    <abstract>Multilocus sequence typing (MLST) based on the 16S RNA, pduF, glnA, and manB
genes was developed for Salmonella, and its discriminatory ability was compared
to those of pulsed-field gel electrophoresis (PFGE) and serotyping. PFGE
differentiated several strains undifferentiable by serotyping, and 78 distinct
PFGE types were identified among 231 Salmonella isolates grouped into 22
serotypes and 12 strains of undetermined serotype. The strains of several PFGE
types were further differentiated by MLST, which suggests that the discriminatory
ability of MLST for the typing of Salmonella is better than that of serotyping
and/or PFGE typing. manB-based sequence typing identified two distinct genetic
clusters containing 32 of 54 (59%) clinical isolates whose manB gene sequences
were analyzed. The G+C contents and Splitstree analysis of the manB, glnA, and
pduF genes of Salmonella indicated that the genes differ in their evolutionary
origins and that recombination played a significant role in their evolution.</abstract>
    <date>2002</date>
    <publication>J Clin Microbiol</publication>
    <refereed>TRUE</refereed>
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